期刊
ANALYTICA CHIMICA ACTA
卷 1111, 期 -, 页码 132-138出版社
ELSEVIER
DOI: 10.1016/j.aca.2020.03.050
关键词
Real-time monitoring; Dynamic changes of intracellular pH; Ionic liquid; Fluorescence probe
资金
- Natural Science Foundation of China [21475017, 21727811]
- Fundamental Research Funds for the Central Universities [N2005027]
Real-time monitoring of intracellular pH is of great significance due to its essential role in physiological and pathological processes. In present work, the ionic liquid (IL) N-methyl-6-hydroxyquinolinium bis(trifluoromethylsulfonyl) imide ([6MQc][NTf2]) is proposed as a fluorescence probe for the quantitative imaging of intracellular pH in response to external stimuli. The fluorescence of the IL [6MQc][NTf2] exhibits a sensitive response to pH variations, as the deprotonation of [6MQc][NTf2] generates the highly fluorescent zwitterionic product [6MQz]. pH fluctuations in the range of 6.0-7.5 can be accurately sensed by monitoring the fluorescence change at 555 nm. Moreover, this IL probe exhibits favorable biocompatibility, excellent anti-photobleaching properties, and high tolerance to ionic strength. Using the IL probe, real-time sensing of hypoxia- and drug-induced intracellular pH changes in MCF-7 cells is achieved. (C) 2020 Elsevier B.V. All rights reserved.
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