期刊
ANALYTICA CHIMICA ACTA
卷 1104, 期 -, 页码 199-206出版社
ELSEVIER
DOI: 10.1016/j.aca.2020.01.017
关键词
SERS; Core-shell; Fe3O4; Histamine; Fish; RAW264.7 cell lysates
资金
- National Natural Science Foundation of China [61575043]
- program for Changjiang Scholars and Innovative Research Team in University [IRT1115]
- Natural Science Foundation of Fujian Province of China [2016J01292]
We have successfully prepared a highly sensitive sandwich nanosensor combined Fe3O4 and Au@ATP@Ag nanorods for histamine detection based on surface-enhanced Raman spectroscopy (SERS). The Fe3O4 beads with -COOH served as a capture part to enrich histamine. The Au@ATP@Ag core-shell nanorods functionalized with Nalpha,Nalpha-Bis(carboxymethyl)-L-lysine (AB-NTA) were then used to connect with the imidazolyl group of histamine, simultaneously the internal standard 4-aminothiophenol (4-ATP) in the core-shell structure was used as the SERS signal. PLS regression model based on concentration range 10(-3)-10(-8)mol/L showed a linear trend with R-2 = 0.9907. Our new approach can quickly and reliably determine histamine in fish sample and RAW264.7 cell lysates. This protocol for histamine extraction and SERS analysis enables the development of ultra-sensitive method for histamine detection. (C) 2020 Elsevier B.V. All rights reserved.
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