期刊
AGING-US
卷 12, 期 7, 页码 5625-5639出版社
IMPACT JOURNALS LLC
DOI: 10.18632/aging.102905
关键词
lncRNA; ceRNA; miR-125a-5p; E2F3; myoblast
资金
- National Natural Science Foundation [31630072, 31972564]
- Breeding of New Varieties of Genetically Modified Pigs with Fodder Saved and high lean meat rate [2016ZX08006001-003]
- Priority Academic Program Development of Jiangsu Higher Education Institutions [DKQB201903]
Although many long non-coding RNAs (lncRNAs) have been identified in muscle, some of their physiological functions and regulatory mechanisms remain elusive. Here we report the functional identification and characterization of a novel lncRNA 2310043L19Rik (lnc-231), which is highly expressed in muscle. The expression level of Inc-231 in skeletal muscle of young mice is higher than that in aged mice. Functional analysis showed that overexpression of lnc-231 restrained differentiation and promoted proliferation of myoblast, while inhibition of lnc-231 revealed completely opposite effects in vitro. RNA molecules of lnc-231 acted mechanistically as competing endogenous RNAs (ceRNA) to target miR-125a-5p, whereas miR-125a-5p binds to the 3'-UTR of E2F3 mRNA to inhibit its function. Collectively, lncRNA 2310043L19Rik promotes proliferation and inhibits differentiation of myoblast cells by attenuating the function of miR-125a-5p.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据