期刊
ADDICTION BIOLOGY
卷 26, 期 2, 页码 -出版社
WILEY
DOI: 10.1111/adb.12889
关键词
alcohol; astrocytes; microglia; neuroimmune; PLX5622; transcriptome
资金
- American Cancer Society [126584-PF-14-216-01-TBF, 128265-RSG-158-01-CSM]
- National Institute on Alcohol Abuse and Alcoholism [AA006399, AA013520, AA020683, AA020926, AA025499, AA012404]
- TAT [126584-PF-14-216-01-TBF]
The depletion of microglia does not affect the sedative or hypnotic effects of acute intoxication, nor does it change the escalation or maintenance of chronic voluntary alcohol consumption, indicating that microglia are not the primary effector cell responsible for regulating alcohol behaviors. Instead, the upregulation of alcohol-responsive, reactive astrocyte genes suggests that astrocytes may play a role in regulating these alcohol behaviors. Activation of microglia through repeated immune stimulation blocks escalations in alcohol intake, indicating that microglia regulate drinking behaviors with sufficient immune activation. This study provides insight into the causal and consequential roles of microglia in the transition from alcohol use to dependence.
Alcohol abuse induces changes in microglia morphology and immune function, but whether microglia initiate or simply amplify the harmful effects of alcohol exposure is still a matter of debate. Here, we determine microglia function in acute and voluntary drinking behaviors using a colony-stimulating factor 1 receptor inhibitor (PLX5622). We show that microglia depletion does not alter the sedative or hypnotic effects of acute intoxication. Microglia depletion also does not change the escalation or maintenance of chronic voluntary alcohol consumption. Transcriptomic analysis revealed that although many immune genes have been implicated in alcohol abuse, downregulation of microglia genes does not necessitate changes in alcohol intake. Instead, microglia depletion and chronic alcohol result in compensatory upregulation of alcohol-responsive, reactive astrocyte genes, indicating astrocytes may play a role in regulation of these alcohol behaviors. Taken together, our behavioral and transcriptional data indicate that microglia are not the primary effector cell responsible for regulation of acute and voluntary alcohol behaviors. Because microglia depletion did not regulate acute or voluntary alcohol behaviors, we hypothesized that these doses were insufficient to activate microglia and recruit them to an effector phenotype. Therefore, we used a model of repeated immune activation using polyinosinic:polycytidylic acid (poly(I:C)) to activate microglia. Microglia depletion blocked poly(I:C)-induced escalations in alcohol intake, indicating microglia regulate drinking behaviors with sufficient immune activation. By testing the functional role of microglia in alcohol behaviors, we provide insight into when microglia are causal and when they are consequential for the transition from alcohol use to dependence.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据