期刊
ACS NANO
卷 14, 期 7, 页码 7868-7879出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsnano.9b09941
关键词
cell protrusion; collagen remodeling; cell-matrix early interaction; dendritic morphology; fibrous traction force microscopy
类别
资金
- NIH [5-T32-HL07081-38]
- NSF Center for Engineering Mechanobiology [CMMI-1548571]
- NCI [U01CA202177, U54CA193417, R01CA232256]
- NIBIB [R01EB017753]
Fibroblasts undergo a critical transformation from an initially inactive state to a morphologically different and contractile state after several hours of being embedded within a physiologically relevant three-dimensional (3D) fibrous collagen-based extracellular matrix (ECM). However, little is known about the critical mechanisms by which fibroblasts adapt themselves and their microenvironment in the earliest stage of cell-matrix interaction. Here, we identified the mechanisms by which fibroblasts interact with their 3D collagen fibrous matrices in the early stages of cell-matrix interaction and showed that fibroblasts use energetically efficient hierarchical micro/nanoscaled protrusions in these stages as the primary means for the transformation and adaptation. We found that actomyosin contractility in these protrusions in the early stages of cell-matrix interaction restricts the growth of microtubules by applying compressive forces on them. Our results show that actomyosin contractility and microtubules work in concert in the early stages of cell-matrix interaction to adapt fibroblasts and their microenvironment to one another. These early stage interactions result in responses to disruption of the microtubule network and/or actomyosin contractility that are opposite to well-known responses to late-stage disruption and reveal insight into the ways that cells adapt themselves and their ECM recursively.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据