期刊
ACS OMEGA
卷 4, 期 25, 页码 21431-21438出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsomega.9b03130
关键词
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资金
- National Natural Science Foundation of China [21535003, 21575022]
- National Key Research and Development Program of China [2017YFA0700404]
- Key Research & Development Plan of Jiangsu Province [BE2018617]
- Qing Lan Project
- Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, MOE, Qingdao University of Science and Technology [STAM201801]
Sensitive detection of severe fever with thrombocytopenia syndrome virus (SFTSV) by a point-of-care assay is of great significance for promoting clinical diagnosis. In this work, ultrasensitive detection of SFTSV was achieved by using fluorescent carbon dots/SiO2 nanospheres (CSNs) as reporters for a lateral flow assay. The prepared CSNs were resistant to extreme environments and had strong stability. The uniform CSNs with the size of about 200 nm were obtained by differential centrifugation. Their absolute quantum yields in the aqueous and solid phases are 56.3 and 36.6%, respectively. The excellent fluorescent properties of CSNs make the test strips more sensitive and have a longer assay lifetime. Thus, the visual detection limit of the lateral flow test strip based on immunofluorescent CSN (iCSN) was as low as 10 pg/mL SFTSV nucleoprotein. The sensitivity of this assay is 2 orders of magnitude higher than that of the colloidal gold-based lateral flow test strip. Besides, the assay owns good reproducibility and high specificity. Then, iCSN-based lateral flow test strips were evaluated in real samples of human serum of patients with satisfactory results. Furthermore, this assay has a general prospect for other fluorescent immunochromatography applications.
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