期刊
PATHOGENS
卷 9, 期 3, 页码 -出版社
MDPI
DOI: 10.3390/pathogens9030160
关键词
antimicrobial activity; BLB; nanotechnology; rice; silver nanoparticles; B. cereus
类别
资金
- PhosAgro/UNESCO/IUPAC/GCUF Project 82 titled Biosynthesis of silver nanoparticles to control bacterial leaf blight of rice
- Zhejiang Provincial Natural Science Foundation of China [LZ19C140002]
- National Natural Science Foundation of China [31872017, 31571971, 31371904, 31801787, 31901925]
- Zhejiang Provincial Key Research and Development Plan [2017C02002, 2019C02006, 2020C02006]
- Key Program of Zhejiang Provincial Foundation for Natural Science [LZ16C130002]
- State Key Laboratory for Quality and Safety of Agro-products [2010DS700124-ZZ1907]
- Science and Technology Project of Ningbo [2016C11017]
- National Key Research and Development Program of China [2018YFD0300900, 2017YFD0201104]
- Shanghai Agriculture Applied Technology Development Program [2019-02-08-00-08-F01150, 2014:7-3-1]
- Fundamental Research Funds for the Central Universities
- Dabeinong Funds for Discipline Development and Talent Training in Zhejiang University
Amongst serious biotic factors deteriorating crop yield, the most destructive pathogen of rice is Xanthomonas oryzae pv. oryzae (Xoo), which causes bacterial leaf blight (BLB) disease. This study involved targeted use of biogenic silver nanoparticles (AgNPs) to control BLB in order to cope with the disadvantages of chemical disease control. AgNPs were biologically synthesized from natively isolated Bacillus cereus strain SZT1, which was identified through 16S rRNA gene sequence analysis. Synthesis of AgNPs in bacterial culture supernatant was confirmed through UV-VIS spectroscopy. Fourier transform infrared spectroscopy (FTIR) confirmed that the existence of AgNPs was stabilized with proteins and alcoholic groups. X-ray diffraction (XRD) data revealed the crystalline nature and imaging with scanning electron microscopy (SEM) and transmission electron microscopy (TEM), showing the spherical shape of AgNPs with particle sizes ranging from 18 to 39 nm. The silver presence in AgNPs was further confirmed by energy dispersive spectra. Biogenic AgNPs showed substantial antibacterial activity (24.21 +/- 1.01 mm) for Xoo. In a pot experiment, AgNPs were found to be effective weapons for BLB by significantly increasing the plant biomass with a decreased cellular concentration of reactive oxygen species and increased concentration of antioxidant enzyme activity.
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