期刊
CARBOHYDRATE RESEARCH
卷 430, 期 -, 页码 48-53出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carres.2016.04.030
关键词
Nitrophenyl beta-D-apiofuranoside; beta-D-Apioftiranosidase; Aspergillus
资金
- Slovak Research and Development Agency [APVV-0846-12]
- Scientific Grant Agency of the Ministry of Education of Slovak Republic
- Slovak Academy of Sciences (VEGA) [2/0138/12, 2/0157/16]
- Research & Development Operational Programmes - ERDF (Centre of Excellence on Green Chemistry Methods and Processes, CEGreenI) [26240120001]
- Research & Development Operational Programmes - ERDF (Amplification of the Centre of Excellence on Green Chemistry Methods and Processes, CEGreenII) [26240120025]
4-Nitrophenyl beta-D-apiofuranoside as a chromogenic probe for detection of fi-n-apiofuranosidase activity was prepared in 61% yield from 2,3-isopropylidene-alpha-beta-u-apiofuranose through a sequence of five reactions. The synthesis involves one regioselective enzymatic step benzoylation of primary hydroxyl of 2,3-isopropylidene-alpha,beta-D-apiofuranose catalysed by Lipolase 100T and stereoselective beta-Dapiofuranosylation of p-nitrophenol using BF3 center dot OEt2/Et3N. The product was used for screening of fi-napiofuranosidase activity in 61 samples of crude commercial enzymes and plant materials. Fifteen enzyme preparations originating from different strains of genera Aspergillns display fi-n-apiofuranosidase activity. The highest activity was found in Rapidase AR 2000 (78.27 U/g) and lyophilized Viscozyme L (64,36 L/g). (C) 2016 Elsevier Ltd. All rights reserved.
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