期刊
ANNALS OF TRANSLATIONAL MEDICINE
卷 7, 期 23, 页码 -出版社
AME PUBL CO
DOI: 10.21037/atm.2019.11.03
关键词
Root resorption; orthodontic tooth movement (OTM); cystathionine gamma lyase (CSE); receptor activator of nuclear factor kappa-B ligand (RANKL)/osteoprotegerin (OPG)
资金
- National Science Foundation of China [81870791]
- Shanghai Science and Technology Committee [17140903700]
Background: This study aimed to determine the contribution of cystathionine gamma lyase (CSE) to physiological and orthodontic root resorption in mice. Methods: Mice genetically deficient in the CSE (CSE-/-), the dominant hydrogen sulfide (H2S)-generating enzyme in osteoclast were used in this study. Physiological and orthodontic root resorption was assessed with micro computed tomography (micro-CT) and scanning electron microscopy (SEM) in the mice at the age of 8-, 26-, and 52-week and in 8-week old mice following 1-, 2-, and 3-week orthodontic treatment, respectively. Hematoxylin and eosin (HE) and tartrate-resistant acid phosphatase (TRAP) staining were used for further determination of root resorption and the number of osteoclasts. The receptor activator of nuclear factor kappa-B ligand (RANKL)/osteoprotegerin (OPG) level in surrounding alveolar bone of the maxillary first molar after 2-week orthodontic treatment was measured by reverse transcription polymerase chain reaction (RT-PCR). Results: Root resorption lacunae (RRL) gradually and significantly increased with age in wild type (WT) and CSE-/- mice. The CSE-/- showed less RRL compared with the WT group. At each time point of orthodontic treatment, the CSE-/- group had less RRL and osteoclasts than the WT group. The orthodontically induced RANKL/OPG mRNA expression in the periodontal tissue in the CSE-/- group was lower than that in the WT group. Conclusions: CSE contributes significantly to physiological and orthodontic root resorption.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据