期刊
MICROORGANISMS
卷 8, 期 1, 页码 -出版社
MDPI
DOI: 10.3390/microorganisms8010079
关键词
probiotic; Lactobacillus casei IMAU60214; health; innate immunity; macrophage; Toll-like receptors
类别
资金
- Fondos Federales Mexico [HIM/2014/013 SSA. 1120]
- E022 Program, National Institute of Pediatrics, Mexico City, Mexico [INP 024/2017, INP 031/2018, INP 038/2019, INP 041/2018]
- CONACYT [259201]
- Catedras CONACYT [2184, 2057]
Most Lactobacillus species have beneficial immunological (immunoprobiotic) effects in the host. However, it is unclear how probiotic bacteria regulate immune responses. The present study investigated the effects of heat-killed Lactobacillus casei IMAU60214 on the activity of human monocyte-derived macrophages (MDMs). Human MDMs were treated with heat-killed L. casei at a ratio (bacteria/MDM) of 50:1, 100:1, 250:1, and 500:1, and then evaluated for the following: NO production, by Griess reaction; phagocytosis of FITC-labeled Staphylococcus aureus particles; cytokine secretion profile (tumor necrosis factor (TNF)-alpha, interleukin (IL)-1 beta, IL-6, IL-12p70, IL-10, and transforming growth factor (TGF)-beta) by ELISA; and costimulatory molecule (CD80 and CD86) surface expression, by flow cytometry. Heat-killed L. casei IMAU60214 enhanced phagocytosis, NO production, cytokine release, and surface expression of CD80 and CD86 in a dose-dependent manner. All products were previously suppressed by pretreatment with a Toll-like receptor 2 (TLR2)-neutralizing antibody. Overall, our findings suggest that this probiotic strain promotes an M1-like pro-inflammatory phenotype through the TLR2 signaling pathway. These effects on macrophage phenotype help explain the probiotic efficacy of Lactobacillus and provide important information for the selection of therapeutic targets and treatments compatible with the immunological characteristics of this probiotic strain.
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