期刊
ANTIOXIDANTS
卷 9, 期 2, 页码 -出版社
MDPI
DOI: 10.3390/antiox9020166
关键词
Anthyllis vulneraria; Fuchsia magellanica; Fuchsia triphylla; Lysimachia nummularia; antimicrobial activity; antioxidant capacity; fibroblasts; keratinocytes; cytotoxicity; cell migration
资金
- NKFIH (Hungarian Scientific Research Fund) [K 127944]
- University of Pecs, Medical School [KA-2018-17]
- project (Comprehensive Development for Implementing Smart Specialization Strategies at the University of Pecs) [EFOP 3.6.1-16-2016-00004]
- Higher Education Institutional Excellence Program of the Ministry for Innovation and Technology in Hungary of the University of Pecs [FIKP II.2.]
Medicinal plants are widely used in folk medicine but quite often their composition and biological effects are hardly known. Our study aimed to analyze the composition, cytotoxicity, antimicrobial, antioxidant activity and cellular migration effects of Anthyllis vulneraria, Fuchsia magellanica, Fuchsia triphylla and Lysimachia nummularia used in the Romanian ethnomedicine for wounds. Liquid chromatography with mass spectrometry (LC-MS/MS) was used to analyze 50% (v/v) ethanolic and aqueous extracts of the plants' leaves. Antimicrobial activities were estimated with a standard microdilution method. The antioxidant properties were evaluated by validated chemical cell-free and biological cell-based assays. Cytotoxic effects were performed on mouse fibroblasts and human keratinocytes with a plate reader-based method assessing intracellular adenosine triphosphate (ATP), nucleic acid and protein contents and also by a flow cytometer-based assay detecting apoptotic-necrotic cell populations. Cell migration to cover cell-free areas was visualized by time-lapse phase-contrast microscopy using standard culture inserts. Fuchsia species showed the strongest cytotoxicity and the highest antioxidant and antimicrobial activity. However, their ethanolic extracts facilitated cell migration, most probably due to their various phenolic acid, flavonoid and anthocyanin derivatives. Our data might serve as a basis for further animal experiments to explore the complex action of Fuchsia species in wound healing assays.
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