期刊
ANTIOXIDANTS
卷 9, 期 1, 页码 -出版社
MDPI
DOI: 10.3390/antiox9010088
关键词
antioxidant activity; peroxyl radicals; fluorescent probes; rate constant; micelles
资金
- National Science Centre, Poland (NCN) [2014/15/B/ST4/04835]
Herein, we describe the synthesis of a fluorescent probe NB-2 and its use for the detection of peroxyl radicals. This probe is composed of two receptor segments (4-hydroxycinnamyl moieties) sensitive towards peroxyl radicals that are conjugated with a fluorescent reporter, dipyrrometheneboron difluoride (BODIPY), whose emission changes depend on the oxidation state of the receptors. The measurement of the rate of peroxidation of methyl linoleate in a micellar system in the presence of 1.0 mu M NB-2 confirmed its ability to trap lipid peroxyl radicals with the rate constant k(inh) = 1000 M-1 center dot s(-1), which is ten-fold smaller than for pentamethylchromanol (an analog of alpha-tocopherol). The reaction of NB-2 with peroxyl radicals was further studied via fluorescence measurements in methanol, with alpha,alpha '-azobisisobutyronitrile (AIBN) used as a source of radicals generated by photolysis or thermolysis, and in the micellar system at pH 7.4, with 2,2 '-azobis(2-amidinopropane) (ABAP) used as a thermal source of the radicals. The reaction of NB-2 receptors with peroxyl radicals manifests itself by the strong increase of a fluorescence with a maximum at 612-616 nm, with a 14-fold enhancement of emission in methanol and a 4-fold enhancement in the micelles, as compared to the unoxidized probe. Our preliminary results indicate that NB-2 behaves as a switch on fluorescent probe that is suitable for sensing peroxyl radicals in an organic lipid environment and in bi-phasic dispersed lipid systems.
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