期刊
CARBOHYDRATE RESEARCH
卷 427, 期 -, 页码 44-47出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carres.2016.03.016
关键词
Escherichia coli; Lipopolysaccharide; O-Polysaccharide; Bacterial polysaccharide structure; O-Antigen gene cluster
资金
- Russian Foundation for Basic Research [14-04-01709]
- National 973 Project of China [2012CB721101, 2013CB733904]
- International Science & Technology Cooperation Program of China [2013DFR30640]
- National Natural Science Foundation of China (NSFC) Program [31530083, 31270003, 81471904, 31371259, 31270133, 31470194]
- Research Project of Chinese Ministry of Education [113015A]
- Swedish Research Council [621-2013-4859]
Mild acid degradation of the lipopolysaccharide of Escherichia coli O132 released its O-polysaccharide. Analysis by 1D and 2D H-1 and C-13 NMR spectroscopy prior and subsequent to O-deacetylation, in conjunction with sugar analysis, revealed a linear pentasaccharide repeating unit of the O-polysaccharide having the following structure: -> 2)-alpha-D-Galf-(1 -> 3)-alpha-L-Rhap2Ac-(1 -> 4)-alpha-D-Glcp-(1 -> 2)-alpha-L-Rhap-(1 -> 3)-beta-D-GlcpNAc-(1 -> Putative functions of genes in the O-antigen gene cluster of E. coli O132 are consistent with the O-polysaccharide structure. (C) 2016 Elsevier Ltd. All rights reserved.
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