期刊
CARBOHYDRATE POLYMERS
卷 153, 期 -, 页码 492-500出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2016.07.124
关键词
Chitosan-collagen hydrogel; PLLA nanofibers; Alginate; Tendon regeneration; Peritendinous adhesion
资金
- Nanomission, Department of Science and Technology (DST), Government of India
- Department of Biotechnology (DBT), India [BT/PR 13585/NNT/28/474/2010]
- Council of Scientific and Industrial Research under the CSIR-SRF award [9/963(0034)2K13-EMR-I]
The aim of our study was to develop a tendon construct of electrospun aligned poly (L-lactic acid) (PLLA) nanofibers, to mimic the aligned collagen fiber bundles and layering PLLA fibers with chitosan-collagen hydrogel, to mimic the glycosaminoglycans of sheath ECM for tendon regeneration. The hydrogel coated electrospun membrane was rolled and an outer coating of alginate gel was given to prevent peritendinous adhesion. The developed constructs were characterized by SEM, FT-IR and tensile testing. Protein adsorption studies showed lower protein adsorption on coated scaffolds compared to uncoated scaffolds. The samples were proven to be non-toxic to tenocytes. The chitosan-collagen/PLLA uncoated scaffolds and alginate gel coated chitosan-collagen/PLLA scaffolds showed good cell proliferation. The tenocytes showed good attachment and spreading on the scaffolds. This study indicated that the developed chitosan-collagen/PLLA/alginate scaffold would be suitable for flexor tendon regeneration. (C) 2016 Elsevier Ltd. All rights reserved.
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