期刊
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
卷 8, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fbioe.2020.00029
关键词
micro-fermentation; beta-carotene; Yarrowia lipolytica; yeast cell factories; metabolic engineering; fermentation; media optimization
资金
- Novo Nordisk Foundation
- Fermentation-based Biomanufacturing Initiative (FBM)
The production of beta-carotene has become increasingly interesting within the biotechnological industry due to a rising demand for safer and more natural colorants, nutritional supplements, and antioxidants. A recent study has described the potential of Yarrowia lipolytica as a beta-carotene-producing cell factory, reporting the highest titer of recombinant beta-carotene produced to date. Finding the best conditions to maximize production and scaling up the process to full scale, a costly and time-consuming process, it is often a bottleneck in biotechnology. In this work, we explored the benefits of using micro-fermentation equipment to significantly reduce the time spent on design and optimization of bioreaction conditions, especially in the early stages of process development. In this proof-of-concept study, a beta-carotene producing Y. lipolytica strain was tested in micro-fermentations partly to assess the robustness of the cell factory design and partly to perform media optimization. The medium optimization led us to an improvement of up to 50% in the yield of beta-carotene production in the best of the conditions. Overall, the micro-fermentation system had a high degree of reliability in all tests.
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