A novel approach for grafting of β-cyclodextrin onto wool via laccase/TEMPO oxidation

This study demonstrated a new enzymatic methodology to graft p-cyclodextrin onto wool. The primary hydroxyl groups in beta-cyclodextrin were oxidized to aldehyde groups using laccase/2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO), which reacted with the amino groups of wool to form Schiff bases. The effects of treatment conditions (treatment temperature, laccase dosage, TEMPO dosage, treatment time) on the aldehyde and carboxyl contents in beta-cyclodextrin were studied. FTIR spectrum of oxidized beta-cyclodextrin showed the presence of aldehyde and carboxyl groups. Results of MALDI-TOF mass spectroscopy confirmed the coupling of beta-cyclodextrin to tyrosine, which was used as a model compound for wool. ATR-FTIR spectroscopy of the grafted wool confirmed the presence of beta-cyclodextrin in grafted wool and the formation of a Schiff base between beta-cyclodextrin and wool. (C) 2016 Elsevier Ltd. All rights reserved.