期刊
CARBOHYDRATE POLYMERS
卷 140, 期 -, 页码 308-313出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2015.12.054
关键词
Gentiana scabra Bunge; Polysaccharides; Purification; Characterization; Anticoagulant activity
资金
- National Natural Science Foundation of China [31171753]
- Nature Science Foundation of Anhui Province [1408085MC52]
- Wuhu's Science and Technology Planning Project [2015hm25]
- Key Technologies R&D Program of Anhui Province [1601031106]
- Students Innovation and Entrepreneurship Training Program [201510363022]
Three water-soluble polysaccharide fractions (GSP-1, GSP-2 and GSP-3) were obtained from Gentiana scabra Bunge roots by DEAE-Sepharose CL-6B and Sepharose CL-6B column chromatography. Their chemical characterizations were determined by high performance gel permeation chromatography (HPGPC), high performance anion exchange chromatography coupled with pulsed amperometric detection (HPAEC-PAD) and Fourier transform infrared (FT-IR) spectrometer. Moreover, their in vitro anticoagulant activities were evaluated by activated partial thromboplastin time (APTT), thrombin time (TT) and prothrombin time (PT) assays. GSP-1 and GSP-2 were composed of rhamnose, arabinose, galactose, glucose and galacturonic acid, while GSP-3 consisted of rhamnose, arabinose, galactose and galacturonic acid with a weight-average molecular weight of 5.8 x 104 Da. In comparison with the control group (saline), GSP, GSP-1, GSP-2 and GSP-3 could prolong APTT and TT, but not PT. Overall, GSP-3 exhibited potent anticoagulant activity and would be expected to be a potential source of anticoagulant. (C) 2015 Elsevier Ltd. All rights reserved.
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