期刊
CARBOHYDRATE POLYMERS
卷 140, 期 -, 页码 144-153出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2015.11.074
关键词
Calcium sulfate; Fibrin nanoparticles; Injectable chitin gel; Angiogenesis; Osteo differentiation; Bone regeneration
资金
- Nanomission, Department of Science and Technology (DST), Government of India
- Council of Scientific and Industrial Research under the CSIR-SRF [9/963(0034)2K13-EMR-I]
Calcium sulfate (CaSO4), an excellent biodegradable bone forming agent that is an ideal choice as additive in gels, however, its disadvantage being poor gel rheology and angiogenesis. Here, we have synthesized chitin-CaSO4-nano-fibrin based injectable gel system which shows improved rheology and angiogenic potential. Rheological studies showed that the composite gel was a shear thinning gel with elastic modulus of 15.4 + 0.275 kPa; a 1.67 fold increase over chitin control. SEM and XRD analyses revealed the effect of nano-fibrin (nFibrin) in transforming CaSO4 crystal shape from needle to hexagonal. It also masked the retarding effect of CaSO4 towards in vitro early cell attachment and angiogenesis using rabbit adipose derived mesenchymal stem cells (rASCs) and HUVECs, respectively. rASCs osteogenesis was confirmed by spectrophotometric endpoint assay, which showed 6-fold early increase in alkaline phosphatase levels and immuno-cytochemistry analysis. These in vitro results highlight the potential of injectable chitin-CaSO4-nFibrin gel for osteo-regeneration via enhanced angiogenesis. (C) 2015 Elsevier Ltd. All rights reserved.
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