期刊
CARBOHYDRATE POLYMERS
卷 140, 期 -, 页码 461-471出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2015.12.053
关键词
Cordyceps gunnii; Extraction met hod; Polysaccharide; Chemical structure; Antitumor activity
资金
- National Spark Key Program of China [2015GA610001]
- Foundation of Tianjin Educational Committee [20090604]
- International Science and Technology Cooperation Program of China [2013DFA31160]
This study was to investigate the effects of different extraction methods on the yield, chemical structure and antitumor activity of polysaccharides from Cordyceps gunnii (C. gunnii) mycelia. Five extraction methods were used to extract crude polysaccharides (CPS), which include room -temperature water extraction (RWE), hot-water extraction (HWE), microwave -assisted extraction (MAE), ultrasound-assisted extraction (UAE) and cellulase-assisted extraction (CAE). Then Sephadex G-100 was used for purification of CPS. As a result, the antitumor activities of CPS and PPS on 5180 cells were evaluated. Five CPS and purified polysaccharides (PPS) were obtained. The yield of CPS by microwave -assisted extraction (CPSMAE) was the highest and its anti -tumor activity was the best and its macromolecular polysaccharide (3000-1000 kDa) ratio was the largest. The PPS had the same monosaccharide composition, but their obvious difference was in the antitumor activity and the physicochemical characteristics, such as intrinsic viscosity, specific rotation, scanning electron microscopy and circular dichroism spectra. (C) 2015 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据