期刊
CARBOHYDRATE POLYMERS
卷 136, 期 -, 页码 826-833出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2015.09.102
关键词
Punctaria plantaginea; Fucan sulfate; Anticoagulant; Antithrombotic; Thrombin; Docking
资金
- Russian Science Foundation [14-50-00126]
Selectively and totally sulfated (1 -> 3)-linked linear homofucans bearing similar to 20 monosaccharide residues on average have been prepared from the branched xylofucan sulfate isolated from the brown alga Punctaria plantaginea. Anticoagulant and antithrombotic properties of the parent biopolymer and its derivatives were assessed in vitro. Highly sulfated linear fucan derivatives were shown to inhibit clot formation in APTT assay and ristocetin induced platelets aggregation, while the partially sulfated analogs were inactive. In the experiments with purified proteins, fucan derivatives with degree of sulfation of similar to 2.0 were found to enhance thrombin and factor Xa inhibition by antithrombin III. The effect of sulfated fucans on thrombin inhibition, which was similar to those of heparinoid Clexane (R) (enoxaparin) and of a fucoidan from the brown alga Saccharina latissima studied previously, can be explained by the multicenter interaction and formation of a ternary complex thrombin-antithrombin III-polysaccharide. The possibility of such complexation was confirmed by computer docking study. (C) 2015 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据