期刊
CARBOHYDRATE POLYMERS
卷 135, 期 -, 页码 162-168出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2015.08.062
关键词
Laminaran; Fucoidan; Alginate; Cocophora langsdorfii; Anticancer activity
资金
- Russian Foundation for Basic Research [14-04-93003]
- FEB of Russian Academy of Sciences [P-42]
Laminaran, fucoidan, and alginate were isolated from the brown alga Coccophora langsdorfii collected in the Japan Sea. The structural characteristics of polysaccharides were investigated by NMR spectroscopy. The laminaran was determined as beta-D-glucan, which consisted of 80% of 1,3- and 20% of 1,6-linked residues and was terminated with mannitol. The alginate was a guluronic acid-rich polysaccharide (M/G = 0.85). Fucoidan, sulfated alpha-L-fucan, contained a linear backbone of alternating (1 -> 3)-and (1 -> 4)- linked alpha-L-fucopyranose residues with sulfate at C2 and C4 of (1 -> 3)-alpha-L-fucopyranose residues. Anticancer activity of this fucoidan was investigated in comparison with activity of fucoidan having similar linear backbone from the brown alga Fucus evanescens. The fucoidan from C. langsdorfii significantly inhibited colony formation of SK-MEL-5 and SK-MEL-28 melanoma cells (the percentage of inhibition was 28 and 76, respectively) and weakly inhibited colony formation of breast adenocarcinoma cells MDA-MB-231 (the percentage of inhibition was about 5). Similar results were obtained for fucoidan from F. evanescens; the percentage of inhibition of colony formation of SK-MEL-5 and SK-MEL-28 melanoma cells was 54 and 56, respectively. The inhibition of colony formation of breast adenocarcinoma cells MDA-MB-231 was weak. We suppose that other sulfated and partially acetylated fucoidans consisting of (1 -> 3)- and (1 -> 4)-linked alpha-L-fucopyranose residues may suppress progression of melanoma cell colony formation similar to fucoidans of C. langsdorfii and F. evanescens. (C) 2015 Elsevier Ltd. All rights reserved.
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