期刊
CARBOHYDRATE POLYMERS
卷 142, 期 -, 页码 199-205出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2016.01.063
关键词
Enzymatic extraction; Pectin composition; Cell wall polysaccharides; Byproducts
Pectins were extracted from apple pomace with monoactive preparation of endo-xylanase and endocellulase. The process was conducted for 10h in conditions of pH 5.0 at 40 C, with constant shaking. Endo-xylanase application resulted in the highest extraction efficiency of pectins (19.8%). The obtained polymer was characterised by a very high molecular mass, high level of neutral sugars - mainly arabinose, galactose and glucose, and very high DM (73.4). It also contained the highest level of protein and phenols. Pectin extracted with endo-cellulase had 1.5 fold lower molecular mass but contained significantly more GalA (70.5%) of a high degree of methylation (66.3%). The simultaneous application of both enzymatic preparations resulted in their cooperation, leading to a decrease of both the extraction efficiency and the molecular mass of pectin. However, this pectin was distinguished by the highest GalA (74.7%) and rhamnose contents. (C) 2016 Elsevier Ltd. All rights reserved.
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