期刊
CARBOHYDRATE POLYMERS
卷 151, 期 -, 页码 78-87出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2016.05.057
关键词
Pullulan; Laccase; Enzymatic cross-linking; Rheology
Carboxymethylpullulan (CMP) has been modified in a two-step grafting reaction of ferulic acid (FA). Acid adipic dihydrazyde (ADH) was first reacted with FA activated with 1-ethyl-3-(3dimethylaminopropyl)carbodiimide hydrochloride (EDC). Then the product of this first reaction was reacted with CMP (activated with EDC). Grafted polysaccharides structure was confirmed by FTIR and 1H NMR spectroscopy. Analyses by size -exclusion chromatography (SEC) coupling on-line with a multi angle light scattering detector (MALS), a viscometer and a differential refractive index detector (DRI) (SECIMALSIDRI/Visco) showed that associations between FA moieties occurred due to hydrophobic interactions. The grafting rates of FA were determined by the Folin-Ciocalteu method and were found between 1.0% and 11.2% (mol/mol anhydroglucose unit). The CMP-FA were then enzymatically cross -linked with laccase from Pleurotus ostreatus. The crosslinking reactions were followed by theological measurements, demonstrating the influence of laccase concentration on kinetics. Elastic modulus and swelling rates of hydrogels depends on FA content only for low values. (C) 2016 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据