期刊
AGRONOMY-BASEL
卷 10, 期 1, 页码 -出版社
MDPI
DOI: 10.3390/agronomy10010087
关键词
alfalfa; Medicago sativa L.; phytoestrogens; agronomic traits; variation; HPLC; cluster analysis
资金
- Program of Continuous scientific work on the creation of new varieties of forage crops at the Agricultural Institute Osijek
Alfalfa is the main perennial legume and a rich source of phytoestrogens. Variation of phytoestrogen content and agronomic traits was evaluated in twenty alfalfa populations over two consecutive years. The most dominant phytoestrogen in alfalfa populations was genistein, while slightly lower levels were found for kaempferol and coumestrol. Several populations (Af 1, 12, 18, 19, 20) showed superior agronomic performance, especially high forage yield (up to 71.93 t ha(-1)). These populations were also characterized by a lower (Af 1, 12, 18, 20) or high (Af 1, 19) content of total and individual phytoestrogens, including Af 8 population with the highest total content (2637.10 mu g g(-1) of DM) and content of most of the individual phytoestrogens and favorable agronomic properties. Ward hierarchical clustering method grouped the twenty evaluated populations into four distinct clusters. Identified materials provide a valuable source of germplasm for further breeding programs in order to develop new high-yielding cultivars with either low (for forage use) or high (for possible pharmaceuticals/nutraceuticals industries) phytoestrogen content.
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