期刊
CARBOHYDRATE POLYMERS
卷 143, 期 -, 页码 246-253出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2016.01.073
关键词
Chitosan; Biomaterials; Biocompatibility; Antibacterial; Zwitterion
资金
- National Natural Science Foundation of China [51573097]
- Natural Science Foundation of Guangdong Province [S2013010013056]
- Science and Technology Project of Guangdong Province [2015A010105033]
- Science and Technology Project of Shenzhen City [JCYJ20120613090936342]
- Nanshan District Key Lab for Biopolymers and Safety Evaluation [KC2014ZDZJ0001A]
Chitosan (CS) has attracted much attention due to its good antibacterial activity and biocompatibility. However, CS is insoluble in neutral and alkaline aqueous solution, limiting its biomedical application to some extent. To circumvent this drawback, we have synthesized a novel N-quaternary ammonium-O-sulfobetaine-chitosan (Q(3)BCS) by introducing quaternary ammonium compound (QAC) and sulfobetaine, and its water-solubility, antibacterial activity and biocompatibility were evaluated compare to N-quaternary ammonium chitosan and native CS. The results showed that by introducing QAC, antibacterial activities and water-solubilities increase with degrees of substitution. The largest diameter zone of inhibition (DIZ) was improved from 0 (CS) to 15 mm (N-Q(3)CS). And the water solution became completely transparent from pH 6.5 to pH 11; the maximal waters-solubility was improved from almost 0% (CS) to 113% at pH 7 (N-Q(3)CS). More importantly, by further introducing sulfobetaine, cell survival rate of Q(3)BCS increased from 30% (N-Q(3)CS) to 85% at 2000 mu g/ml, which is even greater than that of native CS. Furthermore, hemolysis of Q(3)BCS was dropped sharply from 4.07% (N-Q3CS) to 0.06%, while the water solution and antibacterial activity were further improved significantly. This work proposes an efficient strategy to prepare CS derivatives with enhanced antibacterial activity, biocompatibility and water solubility. Additionally, these properties can be finely tailored by changing the feed ratio of CS, glycidyl trimethylammonium chloride and NCO-sulfobetaine. (C) 2016 Elsevier Ltd. All rights reserved.
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