期刊
CARBOHYDRATE POLYMERS
卷 149, 期 -, 页码 163-174出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2016.04.080
关键词
Methacrylated chondroitin sulfate; Thermo-sensitive hydrogel; Photo-polymerization; Shear thinning; Cartilage 3D printing
资金
- European Union's Seventh Framework Programme under project HydroZONES [309962]
- ReumaFonds [LLP-12] Funding Source: researchfish
The aim of this study was to design a hydrogel system based on methacrylated chondroitin sulfate (CSMA) and a thermo-sensitive poly(N-(2-hydroxypropyl) methacrylamide-mono/dilactate)-polyethylene glycol triblock copolymer (M15P10) as a suitable material for additive manufacturing of scaffolds. CSMA was synthesized by reaction of chondroitin sulfate with glycidyl methacrylate (GMA) in dimethylsulfoxide at 50 degrees C and its degree of methacrylation was tunable up to 48.5%, by changing reaction time and GMA feed. Unlike polymer solutions composed of CSMA alone (20% w/w), mixtures based on 2% w/w of CSMA and 18% of M15P10 showed strain-softening, thermo-sensitive and shear-thinning properties more pronounced than those found for polymer solutions based on M15P10 alone. Additionally, they displayed a yield stress of 19.2 +/- 7.0 Pa. The 3D printing of this hydrogel resulted in the generation of constructs with tailorable porosity and good handling properties. Finally, embedded chondrogenic cells remained viable and proliferating over a culture period of 6 days. The hydrogel described herein represents a promising biomaterial for cartilage 3D printing applications. (C) 2016 Elsevier Ltd. All rights reserved.
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