期刊
NPJ VACCINES
卷 5, 期 1, 页码 -出版社
NATURE PORTFOLIO
DOI: 10.1038/s41541-020-0164-y
关键词
-
资金
- USDA NIFA [2015-07821, 2013-67015-21347]
- Animal formula fund [WIS01814]
- Nanovaccine Institute
- NIFA [577462, 2013-67015-21347] Funding Source: Federal RePORTER
Mycobacterium avium subsp. paratuberculosis (M. paratuberculosis) causes Johne's disease in ruminants and is characterized by chronic gastroenteritis leading to heavy economic losses to the dairy industry worldwide. The currently available vaccine (inactivated bacterin in oil base) is not effective in preventing pathogen shedding and is rarely used to control Johne's disease in dairy herds. To develop a better vaccine that can prevent the spread of Johne's disease, we utilized polyanhydride nanoparticles (PAN) to encapsulate mycobacterial antigens composed of whole cell lysate (PAN-Lysate) and culture filtrate (PAN-Cf) of M. paratuberculosis. These nanoparticle-based vaccines (i.e., nanovaccines) were well tolerated in mice causing no inflammatory lesions at the site of injection. Immunological assays demonstrated a substantial increase in the levels of antigen-specific T cell responses post-vaccination in the PAN-Cf vaccinated group as indicated by high percentages of triple cytokine (IFN-gamma, IL-2, TNF-alpha) producing CD8(+) T cells. Following challenge, animals vaccinated with PAN-Cf continued to produce significant levels of double (IFN-gamma, TNF-alpha) and single cytokine (IFN-gamma) secreting CD8(+) T cells compared with animals vaccinated with an inactivated vaccine. A significant reduction in bacterial load was observed in multiple organs of animals vaccinated with PAN-Cf, which is a clear indication of protection. Overall, the use of polyanhydride nanovaccines resulted in development of protective and sustained immunity against Johne's disease, an approach that could be applied to counter other intracellular pathogens.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据