期刊
CARBOHYDRATE POLYMERS
卷 146, 期 -, 页码 187-196出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2016.03.043
关键词
Mulberry fruit; Pectic polysaccharide; Structure; Pre-adipocytes; Apoptosis
资金
- Institute of Chemical Technology in Prague
- Specific University Research (MSMT) [20/2013]
- Gyeonggi-do GRRC program of the Catholic University of Korea [2015-B03]
- APW grant [0305-12]
- VEGA grant [2/0018/15]
A water-soluble polysaccharide JS-MP-1 was isolated from Korean mulberry fruits Oddi (Morus alba L.). Sugar linkage analysis and NMR data confirmed that it is a rhamnogalacturonan type I (RG I) polymer carrying arabinan and arabinogalactan (AG II) side chains. JS-MP-1 reduced dose-dependently the viability of 3T3-L1 pre-adipocyte cells, significantly stimulated the cleavage of caspases 9 and 3 and poly (ADP-ribose) polymerase (PARP) and decreased the ratio of Bcl-2 to Bax expression level that led to mitochondrial dysfunction and apoptosis in pre-adipocyte cells. The apoptotic death was mediated by stimulation of MAPKs (ERK and p38) signalling pathway. These results suggest that JS-MP-1 is able to reduce the number of fat cells and the mass of adipose tissue via inhibition of pre-adipocyte proliferation and thus JS-MP-1 itself or a crude aqueous Oddi extract containing this polysaccharide can be used as functional ingredient of health-beneficial food supplements for the treatment or prevention of obesity disorders. (C) 2016 Elsevier Ltd. All rights reserved.
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