期刊
CELLS
卷 9, 期 1, 页码 -出版社
MDPI
DOI: 10.3390/cells9010104
关键词
YB-1; nuclear translocation; inhibition of transcription; phosphorylation
类别
资金
- Russian Science Foundation [14-14-00879, 19-74-20129, 19-74-10079]
- Russian Foundation for Basic Research [18-34-00359]
- Russian State-Funded Budget Project [VI.57.1.2, 0309-2016-0001]
- Russian Science Foundation [19-74-20129, 14-14-00879, 19-74-10079] Funding Source: Russian Science Foundation
The Y-box binding protein 1 (YB-1) is an RNA/DNA-binding protein regulating gene expression in the cytoplasm and the nucleus. Although mostly cytoplasmic, YB-1 accumulates in the nucleus under stress conditions. Its nuclear localization is associated with aggressiveness and multidrug resistance of cancer cells, which makes the understanding of the regulatory mechanisms of YB-1 subcellular distribution essential. Here, we report that inhibition of RNA polymerase II (RNAPII) activity results in the nuclear accumulation of YB-1 accompanied by its phosphorylation at Ser102. The inhibition of kinase activity reduces YB-1 phosphorylation and its accumulation in the nucleus. The presence of RNA in the nucleus is shown to be required for the nuclear retention of YB-1. Thus, the subcellular localization of YB-1 depends on its post-translational modifications (PTMs) and intracellular RNA distribution.
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