期刊
CARBOHYDRATE POLYMERS
卷 136, 期 -, 页码 1065-1073出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2015.09.108
关键词
Trichoderma pseudokonngii; Exopolysaccharide; Human breast cancer; Apoptosis; Cell cycle arrest
资金
- National Basic Research Program of China [2012CB822102]
- National Natural Science Foundation of China [81370983, 81400864, 81500692]
- Foundation of National Key Scientific Instrument and Equipment Development Projects [2011YQ0301241403]
- Hunan Province Natural Science Key Fund Project [2014SK2003]
- Anhui Provincial Natural Science Foundation [1408085MH197, 1508085MH191]
- Key Program for the Excellent Young Talents in College of Anhui Province [2013SQRL054ZD]
- Natural Science Foundation of Education Department of Anhui Province [KJ2015A199]
- Scientific Research Project of Wannan Medical College [WK201517]
In this study, we reported the anticancer efficacy of exopolysaccharide (EPS) derived from Trichoderma pseudokoningii, on human breast cancer MCF-7 cells. Our results showed that EPS inhibited the proliferation of MCF-7 cells and induced lactic dehydrogenase release by inducing apoptosis and cell arrest at S phase. Further study revealed that EPS-induced apoptosis of MCF-7 cells was associated with alteration of nuclear morphology, disruption of mitochondrial membrane potential and accumulation of intracellular reactive oxygen species. Sequentially, EPS increased the activation of caspase-9 and caspase-3 in a dose-dependent manner; however, caspase-8 remained intact. Western blot analysis revealed that EPS increased the ratio of Bax/Bcl-2 and promoted the release of cytochrome c into the cytoplasm. Taken together, these findings provided evidence that EPS induced the apoptosis of MCF-7 cells through an intrinsic mitochondrial apoptotic pathway and that EPS may therefore be considered as an effective adjuvant agent against human breast cancer. (C) 2015 Elsevier Ltd. All rights reserved.
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