期刊
CANCERS
卷 12, 期 1, 页码 -出版社
MDPI
DOI: 10.3390/cancers12010066
关键词
pediatric brain tumors; liquid biopsy; BRAF; dabrafenib
类别
资金
- 'Fondo de Investigaciones Sanitarias' (FIS) [PI17-01489]
- Miguel Servet Program del Instituto de Salud Carlos III [CP11/00147]
- Ministerio de Economia y Competitividad-FEDERER [RTC-2016-4990-1]
- Premio Fundacion Mutua Madrilena XIII convocatoria, 2016
- Intheos Foundation
Pediatric Central Nervous System (CNS) tumors are the most fatal cancer diseases in childhood. Due to their localization and infiltrative nature, some tumor resections or biopsies are not feasible. In those cases, the use of minimally invasive methods as diagnostic, molecular marker detection, prognostic or monitoring therapies are emerging. The analysis of liquid biopsies which contain genetic information from the tumor has been much more widely explored in adults than in children. We compare the detection of BRAF V600E targetable mutation by digital-PCR from cell-free-DNA and EV-derived DNA (ctDNA) in serum, plasma and cerebrospinal fluid (CSF) isolated from a cohort of 29 CNS pediatric patients. Here we demonstrate that ctDNA isolated from serum and plasma could be successfully analyzed to obtain tumor genetic information which could be used to guide critical treatment decisions.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据