期刊
ACTA PHARMACEUTICA SINICA B
卷 10, 期 8, 页码 1511-1520出版社
INST MATERIA MEDICA, CHINESE ACAD MEDICAL SCIENCES
DOI: 10.1016/j.apsb.2019.12.009
关键词
Aconitum proprietary Chinese medicines; Aconitine; On-line extraction electrospray ionization mass spectrometry; Ambient mass spectrometry; High-performance liquid chromatography-mass spectrometry; Holistic weight toxicity
资金
- National Key R&D Program of China [2018YFC1706104]
- National Natural Science Foundation of China [81603293]
- Young Elite Scientist Sponsorship Program by China Association for Science and Technology (China) [CACM-2018-QNRC1-04]
- Fundamental Research Funds for the Central Public Welfare Research Institutes (China) [ZZ13-YQ-090]
- Key Project at Central Government Level: the ability establishment of sustainable use for valuable Chinese medicine resources (China) [2060302]
- Science and Technology Planning Project at the Department of Science and Technology of Jiangxi Province (China) [20171ACB21046]
Development of rapid analytical methods and establishment of toxic component limitation standards are of great importance in quality control of traditional Chinese medicine. Herein, an online extraction electrospray ionization mass spectrometry (oEESI-MS) coupled with a novel whole process integral quantification strategy was developed and applied to direct determination of nine key aconitine-type alkaloids in 20 Aconitum proprietary Chinese medicines (APCMs). Multi-type dosage forms (e.g., tablets, capsules, pills, granules, and liquid preparation) of APCM could be determined directly with excellent versatility. The strategy has the characteristics of high throughput, good tolerance of matrix interference, small amount of sample (similar to 0.5 mg) and reagent (similar to 240 mu L) consumption, and short analysis time for single sample (<15 min). The results were proved to be credible by high performance liquid chromatography-mass spectrometry (LC-MS) and electrospray ionization mass spectrometry, respectively. Moreover, the limitation standard for the toxic aconitines in 20 APCMs was established based on the holistic weight toxicity (HWT) evaluation and the Chinese Pharmacopoeia severally, and turned out that HWT-based toxicity evaluation results were closer to the real clinical applications. Hence, a more accurate and reliable APCM toxicity limitation was established and expected to play an important guiding role in clinics. The current study extended the power of ambient MS as a method for the direct quantification of molecules in complex samples, which is commonly required in pharmaceutical analysis, food safety control, public security, and many other disciplines. (C) 2020 Chinese Pharmaceutical Association and Institute of Materia Medica, Chinese Academy of Medical Sciences. Production and hosting by Elsevier B.V.
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