期刊
ACS SENSORS
卷 4, 期 12, 页码 3186-+出版社
AMER CHEMICAL SOC
DOI: 10.1021/acssensors.9b01633
关键词
folding aptasensor platform; microfluidic channel; paper-based device; novel nanoassembly; 17 beta-estradiol
资金
- NSFC, China [61673024, 61527815, 61771452, 61775216]
- National Key Research and Development Program, China [2017YFA0205902]
- Key Research programs of Frontier Sciences, CAS, China [QYZDJ-SSW-SYS015]
Owing to its critical role in the development of female reproductive tissues and as a clinical biomarker, there is an urgent need to develop a rapid and cost-effective method to sensitively detect 17 beta-estradiol (E2). In this work, a folding aptasensor platform with microfluidic channels for the label-free electrochemical detection of E2 is described. The platform, designed with a delicate folding structure, integrating filter holes, microfluidic channels, reaction chambers, and a three electrode system, is extremely easy to use. To increase the detection sensitivity and immobilize the aptamer, we synthesized a novel nanoassembly consisting of amine-functionalized single-walled carbon nanotube/new methylene blue/gold nanoparticles (AuNPs) and modified the working electrode with this nanoassembly. The calibration curve obtained from the experimental results exhibited a linear range between 10 pg mL(-1) and 500 ng mL(-1) (R-2 = 0.993), and a detection limit of 5 pg mL-1 was achieved (S/N = 3). Furthermore, experiments to detect E2 in clinical serum were conducted, and the results were highly similar to those obtained using a large electrochemical luminescence apparatus. By integrating multiple functional components, adopting novel nanoassemblies, and using a folding structure, this paper-based platform not only has great potential as a simple and convenient integrated device for point-of-care testing of E2, but also as a portable, low-cost, and highly sensitive aptasensor platform capable of detecting many diagnostic biomarkers with the appropriate aptamers.
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