期刊
ACS SENSORS
卷 5, 期 2, 页码 510-518出版社
AMER CHEMICAL SOC
DOI: 10.1021/acssensors.9b02340
关键词
graphene; label-free detection; refractive index imaging; confocal microscopy; cell differentiation; epithelial-mesenchymal transition
资金
- National Natural Science Foundation of China [61427819, 11774256, 91750205, 61490712, 61605117, 61805157, 81972826]
- Leading Talents Program of Guangdong Province [00201505]
- Natural Science Foundation of Guangdong Province [2016A030312010, 2017A030313351, 2018A030310559]
- Shenzhen Science and Technology Innovation Commission [KQJSCX20170727100838364, KQTD2017033011044403, ZDSYS201703031605029, JCYJ20180507182035270]
- China Postdoctoral Science Foundation [2018M643160]
Label-free imaging and investigation of living cells are significant for many biomedical studies. It has been challenging to detect the epithelial-mesenchymal transition of cells in situ without affecting cellular activity. Here, we present a common-path differential confocal microscope based on the polarization-sensitive absorption of graphene to realize high-performance refractive index imaging and differentiation of living colorectal cancer cells (HCT116) with large detecting depth (1.29 mu m), excellent refractive index resolution (2.86 X 10(-5) RIU), and high spatial resolution (727 nm) simultaneously. Compared with epithelial (parental HCT116) cells, mesenchymal (paclitaxel-resistant HCT116) cells manifest generally lower refractive index values through the refractive index statistics, which is due to the stronger migration ability and weaker surface adherence of mesenchymal cells. The graphene-based microscopy provides an effective label-free approach to high-resolution imaging and study of living cell kinetics, and we expect it to be widely used in the research fields of pathology, tumorigenesis, and chemotherapy.
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