期刊
BIOTECHNOLOGY ADVANCES
卷 33, 期 8, 页码 1671-1684出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.biotechadv.2015.08.002
关键词
Alcohol dehydrogenase/reductase; Enantiopure isomer; Chiral biosynthesis; Directed evolution; Rational design; Modular engineering; Combination methods
资金
- National Science Foundation of China [21336009, 3137010]
- Program for New Century Excellent Talents in Universities [NCET-13-0833]
- Jiangsu Province Basic Research Program [BK20131101]
- Fundamental Research Funds for the Central Universities [JUSRP51409B]
- National Key Basic Research and Development Program of China [2011CB710800]
Alcohol dehydrogenases/reductases predominantly catalyze the asymmetric biosynthesis of optically pure stereoisomers because of their unique chiral constitutions. The enantioselectivities of alcohol dehydrogenases/reductases are substrate- and cofactor-dependent, and therefore they usually catalyze specific reactions with high enantioselectivity under physiological conditions; this may not be suitable for asymmetric biosynthesis with non-natural substrates or non-natural cofactors, and under nonphysiological conditions. It is therefore necessary to modify alcohol dehydrogenases/reductases using various redesigning tools such as directed evolution and rational design, and their combinations, as well as engineering enzyme modules for more efficient production of non-natural products. In this article, progress in these aspects of alcohol dehydrogenase/reductase design is reviewed, and future challenges are discussed. (C) 2015 Elsevier Inc All rights reserved.
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