期刊
ACS CENTRAL SCIENCE
卷 6, 期 2, 页码 155-165出版社
AMER CHEMICAL SOC
DOI: 10.1021/acscentsci.9b00743
关键词
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资金
- NIH [R01EB024014, R01EB025985, R03AI138189]
- DOD [A132172]
- UCSF Resource Allocation Program
Incorporation of D-amino acids into peptidoglycan is a unique metabolic feature of bacteria. Since D-amino acids are not metabolic substrates in most mammalian tissues, this difference can be exploited to detect living bacteria in vivo. Given the prevalence of D-alanine in peptidoglycan muropeptides, as well as its role in several antibiotic mechanisms, we targeted this amino acid for positron emission tomography (PET) radiotracer development. D-[3-C-11]Alanine and the dipeptide D-[3-C-11]alanyl-D-alanine were synthesized via asymmetric alkylation of glycine-derived Schiff-base precursors with [C-11]methyl iodide in the presence of a cinchonidinium phase-transfer catalyst. In cell experiments, both tracers showed accumulation by a wide variety of both Gram-positive and Gram-negative pathogens including Staphylococcus aureus and Pseudomonas aeruginosa. In a mouse model of acute bacterial myositis, D-[3-C-11]alanine was accumulated by living microorganisms but was not taken up in areas of sterile inflammation. When compared to existing clinical nuclear imaging tools, specifically 2-deoxy-2-[F-18]fluoro-D-glucose and a gallium citrate radiotracer, D-alanine showed more bacteria-specific uptake. Decreased D-[3-C-11]alanine uptake was also observed in antibiotic-sensitive microbes after antimicrobial therapy, when compared to that in resistant organisms. Finally, prominent uptake of D-[3-C-11]alanine uptake was seen in rodent models of discitis-osteomyelitis and P. aeruginosa pneumonia. These data provide strong justification for clinical translation of D-[3-C-11]alanine to address a number of important human infections.
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