期刊
CELL SYSTEMS
卷 10, 期 2, 页码 213-+出版社
CELL PRESS
DOI: 10.1016/j.cels.2020.01.003
关键词
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资金
- Horizon 2020 Marie Sklodowska-Curie Action ITN 2017 of the European Commission [765502-A4B]
- Horizon 2020 programme of the European Union [823839]
- BMBF (de.NBI) [031A535A]
Top-down mass spectrometry (TD-MS)-based proteomics analyzes intact proteoforms and thus preserves information about individual protein species. The MS signal of these high-mass analytes is complex and challenges the accurate determination of proteoform masses. Fast and accurate feature deconvolution (i.e., the determination of intact proteoform masses) is, therefore, an essential step for TD data analysis. Here, we present FLASHDeconv, an algorithm achieving higher deconvolution quality, with an execution speed two orders of magnitude faster than existing approaches. FLASHDeconv transforms peak positions (m/z) within spectra into log m/z space. This simple transformation turns the deconvolution problem into a search for constant patterns, thereby greatly accelerating the process. In both simple and complex samples, FLASHDeconv reports more genuine feature masses and substantially fewer artifacts than other existing methods. FLASHDeconv is freely available for download here: https://www.openms.org/flashdeconv/. A record of this paper's Transparent Peer Review process is included in the Supplemental Information.
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