Green synthesis of cobalt oxide nanoparticles for potential biological applications

Cobalt oxide nanoparticles (Co3O4-Nps) have many applications and now a days the green methods of synthesis of these NPs are preferred over other methods because of associated benefits. In this study, Co3O4-Nps were synthesized by using leaves extract of Populus ciliata (safaida) and cobalt nitrate hexa hydrate as a source of cobalt. The synthesized NPs were analyzed by different techniques such as fourier transform spectroscopy (FTIR), x-ray diffraction (XRD), transmission electron microscopy (TEM) and scanning electron microscopy (SEM). Antibacterial activities of the synthesized Co3O4-Nps were evaluated against gram negative and gram positive bacteria and found active against Escherichia coli (E. coli), Klebseilla pneumonia (K. pneumonia), Bacillus subtillus (B.subtillus) and Bacillus lichenifermia (B. lichenifermia). The activity results were analyzed statistically by one-way ANOVA, with 'Dunnett's Multiple Comparison Test'. The maximum mean activity (21.8 0.7) was found for B. subtilis and minimum mean activity (14.0 0.6) was observed for E. coli.