期刊
JOURNAL OF FORESTRY RESEARCH
卷 32, 期 2, 页码 779-787出版社
NORTHEAST FORESTRY UNIV
DOI: 10.1007/s11676-019-01086-6
关键词
Bursaphelenchus xylophilus; Bx-MRP gene family; Gene and protein structure; Bioinformatics; Multidrug stress
类别
资金
- National Key Research and Development Program of China [2017YFD0600101]
Genes homologous to members of the MRP gene family in pine wood nematode are identified and found to play important roles in drug resistance. Experimental results show that the expression of Bx-MRP family members increases after treatment with different drugs, indicating a positive role in the regulation of multidrug resistance.
Genes homologous to members of the MRP gene family in Caenorhabditis elegans are important in drug resistance. To further explore the molecular mechanism of drug resistance in pine wood nematode (Bursaphelenchus xylophilus), we used bioinformatics approaches to analyze genomic data for B. xylophilus and identified Bx-MRP genes. We predicted the structure and function of the genes and encoded proteins. Using bioinformatics programs to predict and analyze various properties of the predicted proteins, including hydrophobicity, transmembrane regions, phosphorylation sites, and topologically isomeric structures, of these Bx-MRP genes, we determined that they function in transmembrane transport. From the results of RT-qPCR, the Bx-MRP family members confer significant differential resistance to different drug treatments. After treatment with different concentrations of emamectin benzoate, avermectin and matrine, the expression of each gene increased with increasing drug concentrations, indicating that the family members play a positive role in the regulation of multidrug resistance.
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