期刊
APPLIED SCIENCES-BASEL
卷 10, 期 3, 页码 -出版社
MDPI
DOI: 10.3390/app10030768
关键词
biological 3D printing; sodium alginate; gelatin; cartilage scaffold
类别
资金
- National Natural Science Foundation of China [51805475, 51675148, 51275141, 51305113]
- Key Discipline of The Ocean Techtronic Equipment's Technology
- Nature Science Foundation of Zhejiang Provinces Technology 101 [51475129]
- 3D assembly of complex biological structure [LY14E050026]
- Zhejiang Province Higher Visiting Scholar project Technology 101 [51475129]
- Malaysia's Ministry of Higher Education [DIP-2017-001]
- Outstanding Young Teachers Fund of Hangzhou Dianzi University [GK160203201002/003]
The main aim of this paper is to achieve the suitable SA-GEL (sodium alginate and gelatin) porous cartilage scaffold by 3D printing technology with optimal prediction parameters. Firstly, the characteristics of SA-GEL were analyzed, the influence of calcium chloride on the gel was explored, and the optimal cross-linking concentration and gelation temperature were determined. Secondly, a prediction model of the extrusion line width of SA-GEL was established, in which the printing pressure, the moving speed of the needle and the fiber interval were the important parameters affecting the printing performance of the SA-GEL composite material. Thirdly, the SA-GEL composite scaffolds were printed on the Bio-plotter platform, the C5.18 chondrocytes cells were cultured in the SA-GEL biomaterial scaffold, and the results show that the cells could survive well. These results show that, under the control of the printing parameters pressure 1.8 bar, moving speed 10.7 mm/s and the internal structure parameters of the scaffold is 0/45-1.2 (Printing interval: 1.2 mm, angle value: 45 degree), SA-GEL scaffold printing results can be obtained which have good mechanical properties and biocompatibility.
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