期刊
ACS BIOMATERIALS SCIENCE & ENGINEERING
卷 6, 期 1, 页码 225-234出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsbiomaterials.9b01156
关键词
carbon scaffolds; biomaterials; neural stem cells; dopamine detection; stem cell differentiation
资金
- National Science Foundation Major Research Instrumentation Program [CHE-1338173]
- UC Irvine Institute for Design and Manufacturing Innovation
Advances in stem-cell therapy rely on new, multifunctional smart scaffolds (MSS) to promote growth while simultaneously characterizing stem cells undergoing selective differentiation. Nondestructive cell characterization techniques, such as electrochemical detection of lineage-specific metabolites, play a critical role in translational stem-cell therapy by providing clinicians with real-time information to evaluate cell-readiness for transplant. However, electrochemical sensors that provide biophysical cues capable of guiding cell fate, while preserving electroactive functionality, remain unavailable. In this work, a carbon MSS is fabricated by pyrolyzing polyacrylonitrile (PAN) with optimal multiwalled carbon nanotube (MVVCNT) loading to optimize electrochemical activity and with a tunable surface to promote cell growth and organization. Carbon MSS is used to (1) enhance the morphology and differentiation of mouse neural stem/progenitor cells (mNSPCs) derived from different regions of the developing brain and (2) simultaneously detect a neurotransmitter, dopamine, from a model dopaminergic cell line growing on the electrode. The study presents a carbon multifunctional smart scaffold for advancing stem-cell therapy toward clinically relevant applications.
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