期刊
ACS BIOMATERIALS SCIENCE & ENGINEERING
卷 6, 期 1, 页码 575-586出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsbiomaterials.9b01381
关键词
high porosity; freeze-dried; three-dimensional scaffold; osteogenic differentiation
资金
- Natural Science Foundation of Jiangsu Province [BK20171174]
- China Postdoctoral Science Special Foundation [2019T120463]
Three-dimensional honeycomb porous carbon (HPC) has attracted increasing attention in bioengineering due to excellent mechanical properties and a high surface-to-volume ratio. In this paper, a three-dimensional chitosan (CS)/honeycomb porous carbon/hydroxyapatite composite was prepared by nano-sized hydroxyapatite (nHA) on the HPC surface in situ deposition, dissolved in chitosan solution, and vacuum freeze-dried. The structure and composition of CS/HPC/nIIA were characterized by scanning electron microscopy, transmission electron miscroscopy, Fourier transform infrared, and X-ray photo-electron spectroscopy, and the porosity, swelling ratio, and mechanical properties of the scaffold were also tested. The as-prepared scaffolds possess hierarchical pores and organic inorganic components, which are similar in composition and structure to bone tissues. The synthesized composite scaffold has high porosity and a certain mechanical strength. By culturing mouse bone marrow mesenchymal stem cells on the surface of the scaffold, it was confirmed that the scaffold facilitated its growth and promoted its differentiation into the osteogenesis direction. In vivo experiments further demonstrate that the CS/HPC/nHA composite scaffold has a significant advantage in promoting bone formation in the bone defect area. All the results suggested that the CS/HPC/nHA scaffolds have great application prospect in bone tissue engineering.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据