期刊
BIOTECHNIQUES
卷 59, 期 6, 页码 368-371出版社
BIOTECHNIQUES OFFICE
DOI: 10.2144/000114364
关键词
ancient DNA; paleogenomics; DNA library preparation; next-generation sequencing
资金
- Lundbeck Foundation [R52-A5062]
- Danish Council for Independent Research [10-081390]
- Danish National Research Foundation [DNRF94]
- Marie Curie Actions FP7 People [290344]
- Marie Curie Actions Intra-European Fellowship [FP7-PEOPLE-2011-IEF-298820]
- [FP7-IEF-328024]
- Direct For Biological Sciences [1640788] Funding Source: National Science Foundation
- Division Of Environmental Biology [1640788] Funding Source: National Science Foundation
An innovative single-stranded DNA (ssDNA) library preparation method has sparked great interest among ancient DNA (aDNA) researchers, especially after reports of endogenous DNA content increases >20-fold in some samples. To investigate the behavior of this method, we generated ssDNA and conventional double-stranded DNA (dsDNA) libraries from 23 ancient and historic plant and animal specimens. We found ssDNA library preparation substantially increased endogenous content when dsDNA libraries contained <3% endogenous DNA, but this enrichment is less pronounced when dsDNA preparations successfully recover short endogenous DNA fragments (mean size < 70 bp). Our findings can help researchers determine when to utilize the time-and resource-intensive ssDNA library preparation method.
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