期刊
BIOTECHNIQUES
卷 59, 期 1, 页码 42-45出版社
BIOTECHNIQUES OFFICE
DOI: 10.2144/000114310
关键词
long mate pair (LMP) construction; insert sizes; genome assembly; wheat
资金
- BBSRC Triticeae Genomics for Sustainable Agriculture Grant [BB/J003743/1]
- BBSRC National Capability Grant [BB/J010375/1]
- BBSRC [BBS/E/T/000PR5885, BB/J003743/1, BBS/E/T/000PR6193] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BBS/E/T/000PR6193, BB/J003743/1, BBS/E/T/000PR5885] Funding Source: researchfish
Long mate pair (LMP) or jump libraries are invaluable for producing contiguous genome assemblies and assessing structural variation. However the consistent production of high quality (low duplication rate, accurately sized) LMP libraries has proven problematic in many genome projects. Input DNA length and quantity are key issues that can affect success. Here we demonstrate how 12 libraries covering a wide range of jump sizes can be constructed from <10 mu g of DNA, thus ensuring production of the best LMP libraries from a given DNA sample. Finally, we demonstrate the accuracy of the insert sizes by mapping reads from each library back to an existing assembly.
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