期刊
MICROMACHINES
卷 11, 期 2, 页码 -出版社
MDPI
DOI: 10.3390/mi11020186
关键词
in situ PCR; molecular diagnosis; microfluidic chip; POC device; ZIKV
类别
资金
- Class C talent fund project of Chinese Academy of Sciences [CXJJ-19-C03]
- Dean's Fund of Hefei Institute of Physical Science, China [YZJJ2018QN22]
- Open Subject of Key Laboratory of Optoelectronic Materials Science and Technology of Anhui [OMST201701]
- Key Research and Development Program of Anhui [1804a09020051, 1804c04020335, 201904c03020001]
- Youth Innovation Promotion Association of the Chinese Academy of Sciences [2018489]
The outbreak of Zika virus (ZIKV) has posed a great challenge to public health in recent years. To address the urgent need of ZIKV RNA assays, we integrate the microfluidic chip embedded with chitosan-modified silicon dioxide capillaries, smartphone-based detection unit to be a C-3-system for the rapid extraction and detection of ZIKV RNA. The C-3-system is characterized by: (1) four chitosan-modified silicon dioxide capillaries integrated in the microfluidic chip for target ZIKV RNA enrichment and in situ PCR (polymerase chain reaction) amplification; (2) smartphone-based point of care (POC) device consisting of a pneumatic subsystem for controlling the nucleic acid extraction processes in the microfluidic chip, a heating subsystem for sample lysis and PCR amplification, and an optical subsystem for signal acquisition. The entire detection processes including sample lysis, ZIKV RNA enrichment, and reverse-transcription polymerase chain reaction (RT-PCR) is achieved in the microfluidic chip. Moreover, PCR buffers can be directly loaded into the chitosan-modified silicon dioxide capillaries for in situ PCR, in which the captured ZIKV RNA is directly used for downstream PCR without any loss. ZIKV RNA extracted by the C-3-system can be successfully recovered at very low concentrations of 50 transducing units (TU)/mL from crude human saliva. This means that our method of detecting viremia in patients infected with ZIKV is reliable.
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