期刊
FRONTIERS IN MICROBIOLOGY
卷 10, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fmicb.2019.03122
关键词
Bacillus subtilis; nisin; germination; activation; mild treatments
类别
资金
- French National Research Agency (ANR) [ANR-14-CE20-0004]
- Bourgogne-Franche-Comte Regional Council
- European Regional Development Fund (FEDER)
- Agence Nationale de la Recherche (ANR) [ANR-14-CE20-0004] Funding Source: Agence Nationale de la Recherche (ANR)
Bacterial spores are extremely resistant life-forms that play an important role in food spoilage and foodborne disease. The return of spores to a vegetative cell state is a three-step process, these being activation, germination, and emergence. High-pressure (HP) processing is known to induce germination in part of the spore population and even to inactivate a high number of Bacillus spores when combined with other mild treatments such as the addition of nisin. The aim of the present work was to investigate the mechanisms involved in the sensitization of spores to nisin following HP treatment at ambient temperature or with moderate heating leading to a heterogeneous spore response. Bacillus subtilis spores were subjected to HP treatment at 500 MPa at 20 and 50 degrees C. The physiological state of different subpopulations was characterized. Then Fourier transform infrared (FTIR) microspectroscopy coupled to a synchrotron infrared source was used to explore the heterogeneity of the biochemical signatures of the spores after the same HP treatments. Our results confirm that HP at 50 degrees C induces the germination of a large proportion of the spore population. HP treatment at 20 degrees C generated a subpopulation of ungerminated spores reversibly sensitized to the presence of nisin in their growth medium. Regarding infrared spectra of individual spores, spores treated by HP at 50 degrees C and germinated spores had similar spectral signatures involving the same structural properties. However, after HP was performed at 20 degrees C, two groups of spores were distinguished; one of these groups was clearly identified as germinated spores. The second group displayed a unique spectral signature, with shifts in the spectral bands corresponding to changes in membrane fluidity. Besides, spores spectra in the amide region could be divided into several groups close to spectral properties of dormant, germinated, or inactivated spores. The part of the spectra corresponding to alpha-helix and beta-sheet-structures contribute mainly to the spectral variation between spores treated by HP at 20 degrees C and other populations. These changes in the lipid and amide regions could be the signature of reversible changes linked to spore activation.
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