期刊
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
卷 8, 期 11, 页码 4341-4345出版社
AMER CHEMICAL SOC
DOI: 10.1021/acssuschemeng.0c00058
关键词
Biobased chemicals; Biocatalysis; Furans; 5-Hydroxymethylfurfural; Oxidation; Oxidoreductases
资金
- National Natural Science Foundation of China [21676103]
- Natural Science Foundation of Guangdong Province [2017A030313056]
- Guangzhou Municipal Science and Technology Project [201804010179]
2,5-Furandicarboxylic acid (FDCA) is a promising biobased building block for the synthesis of polymers. In this work, a novel whole-cell biocatalyst was constructed by coexpressing vanillin dehydrogenase (VDH1) and HMF/furfural oxidoreductase (HmfH) in Escherichia coli for cascade catalytic oxidation of 5-hydroxymethylfurfural (HMF) to FDCA under sacrificial substrate-free conditions. HMF was rapidly transformed to 5-hydroxymethyl-2-furancarboxylic acid (HMFCA) by VDH1, followed by subsequent oxidation to 5-formyl-2-furancarboxylic acid (FFCA) by HmfH, and finally, FFCA was converted to FDCA by VDH1 and/or HmfH. Under pH-controlled conditions, the biocatalyst enabled efficient synthesis of FDCA from 150 mM HMF in a 96% yield. Besides, FDCA was prepared in the gram scale with a productivity of around 0.4 g/L h.
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