4.6 Article

Dynamic trafficking and turnover of JAM-C is essential for endothelial cell migration

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PLOS BIOLOGY
卷 17, 期 12, 页码 -

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PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pbio.3000554

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资金

  1. MRC [MR/M019179/1]
  2. People Programme (Marie Curie Actions) of the European Union's Seventh Framework Programme (FP7/2007-2013) under REA [608765]
  3. Wellcome Trust [098291/Z/12/Z]
  4. Canceropole PACA (Valo-Paca 2016)
  5. French National Institute of Cancer (Inca, PRT-K16) [2017-24]
  6. BBSRC [BB/M006174/1]
  7. Barts and The London Charity [297/2249]
  8. MRC LMCB core grant [MC_U12266B]
  9. QMUL
  10. BBSRC [BB/M006174/1] Funding Source: UKRI
  11. MRC [MR/M019179/1] Funding Source: UKRI

向作者/读者索取更多资源

Junctional complexes between endothelial cells form a dynamic barrier that hinders passive diffusion of blood constituents into interstitial tissues. Remodelling of junctions is an essential process during leukocyte trafficking, vascular permeability, and angiogenesis. However, for many junctional proteins, the mechanisms of junctional remodelling have yet to be determined. Here, we used receptor mutagenesis, horseradish peroxidase (HRP), and ascorbate peroxidase 2 (APEX-2) proximity labelling, alongside light and electron microscopy (EM), to map the intracellular trafficking routes of junctional adhesion molecule-C (JAM-C). We found that JAM-C cotraffics with receptors associated with changes in permeability such as vascular endothelial cadherin (VE-Cadherin) and neuropilin (NRP)-1 and 2, but not with junctional proteins associated with the transmigration of leukocytes. Dynamic JAM-C trafficking and degradation are necessary for junctional remodelling during cell migration and angiogenesis. By identifying new potential trafficking machinery, we show that a key point of regulation is the ubiquitylation of JAM-C by the E3 ligase Casitas B-lineage lymphoma (CBL), which controls the rate of trafficking versus lysosomal degradation.

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