期刊
G3-GENES GENOMES GENETICS
卷 10, 期 4, 页码 1353-1360出版社
GENETICS SOCIETY AMERICA
DOI: 10.1534/g3.120.401133
关键词
CRISPR; Cas9; ovary translocation; reverse genetics
资金
- NSF/BIO [1645331]
- NIH/NIAID [R21AI111175, R01AI128201, R21AI138074, R21AI129507, R01AI29746]
- USDA/NIFA [2014-10320, 2017-67012-26101]
- USDA Hatch funds [1010032, PEN04608]
- Pennsylvania Department of Health using Tobacco Settlement Funds
- Wolfson Foundation
- Royal Society fellowship [RSWF\R1\180013]
- John S. Dunn Foundation Collaborative Research Award
- Fulbright Fellowship
- Colciencias
Innovative tools are essential for advancing malaria control and depend on an understanding of molecular mechanisms governing transmission of malaria parasites by Anopheles mosquitoes. CRISPR/Cas9-based gene disruption is a powerful method to uncover underlying biology of vector-pathogen interactions and can itself form the basis of mosquito control strategies. However, embryo injection methods used to genetically manipulate mosquitoes (especially Anopheles) are difficult and inefficient, particularly for non-specialist laboratories. Here, we adapted the ReMOT Control (Receptor-mediated Ovary Transduction of Cargo) technique to deliver Cas9 ribonucleoprotein complex to adult mosquito ovaries, generating targeted and heritable mutations in the malaria vector Anopheles stephensi without injecting embryos. In Anopheles, ReMOT Control gene editing was as efficient as standard embryo injections. The application of ReMOT Control to Anopheles opens the power of CRISPR/Cas9 methods to malaria laboratories that lack the equipment or expertise to perform embryo injections and establishes the flexibility of ReMOT Control for diverse mosquito species.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据