Influenza Reverse Genetics-Historical Perspective

The generation of wild-type, mutant, and reassortant influenza viruses from viral cDNAs (reverse genetics) is now a basic molecular virology technique in many influenza virus laboratories. Here, I describe the original RNA polymerase I reverse genetics system and the modifications that have been developed in past years. Together, these technologies have made possible many advances in basic and applied influenza virology that would not have been otherwise attainable, including the revival and study of extinct influenza viruses, the rapid characterization of emerging influenza viruses, the generation of conventional influenza vaccines, and the development of novel influenza vaccines.

Automated summary

Reverse genetics has become a fundamental technique in many influenza virus laboratories, allowing for advancements in both basic and applied virology studies. This technology enables the study and revival of extinct influenza viruses, rapid characterization of new influenza strains, as well as the generation of traditional and novel influenza vaccines.